Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride

CAS Number: 1185-53-1

Linear Formula: NH₂C(CH₂OH)₃ · HCl

Molecular Weight: 157.60

Beilstein Registry Number: 3675235

EC Number: 214-684-5

MDL Number: MFCD00012590

PubChem Substance ID: 24889855

Other Notes
_x000D_ Component of extraction buffer e.g. phenol extraction of DNA or RNA; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE.

Application
_x000D_ The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
_x000D_ For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma hydrochloride is used as a general reagent for the preparation of all types of Tris buffers.

Legal Information
_x000D_ Trizma is a registered trademark of Sigma-Aldrich Co. LLC