Storage: 2-8°C

UNSPSC Code: 12352200

RIDADR: NONH for all modes of transport

Analysis Note:
No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.

Application:
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled compounds using:
€¢ ELISA
€¢ Immunohistocytochemistr<wbr>y
€¢ In situ hybridization
€¢ Western blot
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General description:
Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.

Other Notes:
For life science research only. Not for use in diagnostic procedures.

Preparation Note:
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline: €¢ ELISA: for coating: 2 to 4 μg/ml
€¢ Immunohistocytochemistr<wbr>y: 0.5 to 2 μg/ml
€¢ In situ hybridization: 0.2 to 0.4 μg/ml
€¢ Western blot: 0.5 to 2 μg/ml

Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4</wbr>

Reconstitution:
Add 1 ml PBS to a final concentration of 200 μg/ml.

Specificity:
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.