Storage: 2-8°C

UNSPSC Code: 12352200

RIDADR: NONH for all modes of transport

Application:
Anti-Digoxigenin-Fluoresc<wbr>ein, Fab fragments are used for the detection of digoxigenin-labeled compounds using:
€¢ Digoxigenin-labeled sugars in glycoconjugate research
€¢ Fluorescent in situ hybridization (FISH)
€¢ Immunohistocytochemistr<wbr>y
€¢ In situ hybridization
Anti-Digoxigenin-Fluoresc<wbr>ein, Fab fragments cannot be used for membrane applications.</wbr></wbr></wbr>

General description:
Anti-Digoxigenin-Fluoresc<wbr>ein, Fab fragments has fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to fluorescein.</wbr>

Other Notes:
For life science research only. Not for use in diagnostic procedures.

Preparation Note:
€¢ Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:Detection of digoxigenin-labeled sugars in glycoproteins: 50 to 20μg/ml
€¢ Fluorescent in situ hybridization (FISH): 1 to 20μg/ml
€¢ Immunohistocytochemistry: 20 to 50μg/ml
€¢ In situ hybridization: 20 to 50μg/ml

The anti-DIG anitbody is highly specific and sensitive. Therefore, for FISH, a concentration of 1μg/ml is sufficient, and only in case of critical probes, this concentration may be raised to <10μg/ml (with a distinct background to be expected).
Working solution: Reconstitution
Add 1 ml double-distilled water to a final concentration of 200μg/ml.

Blocking:
PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore, pH can be increased up to pH 8.5 to 9.0.
Storage conditions (working solution): Always prepare fresh!

Reconstitution:
Add 1 ml double-distilled water to a final concentration of 200 μg/ml.

Specificity:
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.