Synonyms: CIAP; CIP; Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

CAS Number: 9001-78-9

MDL No.: MFCD00131849

Storage:20C

Enzyme Commission (EC) Number: 3.1.3.1  ( BRENDA | IUBMB )

UNSPSC Code: 12352204

General description: Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5'-OH can be phosphorylated with polynucleotide kinase and gamma32P-ATP to produce gamma32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

Application: Commonly used to remove the 5'-terminal phosphate from nucleic acids during molecular cloning reactions.

Components: Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl2, and 0.1 mM ZnCl2, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.

Unit Definition: One unit will hydrolyze 1 mumole of p-nitrophenyl phosphate per min at 37 C.

Other Notes: Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 C for 10 minutes.