Storage:20C

UNSPSC Code: 12352200

General description: Sigma's pBR322 Hae III Digest contains 22 fragments from 8 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application: Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Components: Sigma's pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Preparation Note: Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.

Other Notes: For optimal resolution, the recommended agarose gel concentration is 2.5.

Other Notes: Contains 22 fragments, 8-587 bp