PCR Products on a NuSieve™ 3:1 Agarose Gel €“ A 550 bp sequence from lambda DNA was amplified (25 cycles) using primers and Taq DNA polymerase supplied in the GeneAmp® kit (Roche Molecular Systems). PCR products and controls were electro phoresed on a 4% NuSieve™ 3:1 agarose gel in TAE buffer at 5 V/cm for 3 hours. Lane 1, Msp I digest of pBR322 DNA (1.5 μg); lane 2, Hae III digest of ׸X174 DNA (1.5 μg); lane 3, no DNA control; lanes 4 €“ 9, PCR products resulting from different reaction conditions (7 μl of 100 μl reaction mixture); and lane 10, a positive control where kit template was added.