Lambda DNA: Lambda DNA is used as one of the substrates in restriction enzymes research and in testing of restriction enzymes activity.  Phage is isolated from heat-inducible lysogen E. coli W3350 (c/857 Sam 7) strain.  DNA is isolated from purified phage by phenol extraction and dialyzed against 10mM Tris-HCl (pH 7.6) and 1mM EDTA. It contains 48502 bases pairs.