G-Sep™ Q Agarose Fast Flow (FF) resin is a strong anion exchanger composed of highly cross-linked 6% agarose beads, with quaternary ammonium (Q) strong anion exchange groups.

The resin is extensively used for biomacromolecule separations in both research and industrial applications. The G-Sep™ Q Agarose Fast Flow (FF) resins have high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols and have been the industrial stand ard for ion exchange chromatography during recent decades.

Other G-Sep™ Ion Exchange resins include the weak anion exhangerDEAE, the weak cation exhangerCM and the strong cation exhangerSP.

Features

• Matrix: Cross-linked agarose beads, 6%
• Ligand : Quaternary ammonium
• Ion Exchanger : Strong anion exchanger
• Bead form: Spherical, diameter 50-160μm
• Ionic Capacity: 0.18-0.25mmol (Cl^-)/ml
• Binding Capacity: 120mg HSA/ml medium
• pH stability Working Range: 2-12
• pH stability Cleaning-in-Place (CIP): 2-14
• Maximum Flow Velocity: 450cm/h
• Maximum Pressure: 0.3MPa
• Exclusion limit(globular proteins): 4 x 10⁶
• Physical Stability: Negligible volume variation due to changes in pH or ionic strength
• Chemical Stability: Stable to all commonly used aqueous buffers:1M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
• Autoclavable: With Cl^- as counter Ions, at 121°C, pH 7, for 30 min
• Storage Conditions: 4 to 30°C, 20% Ethanol

Applications

• For Ion Exchange Chromatography