Preparation Method
_x000D_ Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in CIRCLEGROW formulations. Chosen reagents are combined in the accurate proportions according to the formula, milled and thoroughly blended for a uniform distribution.

Applications
_x000D_ Circlegrow® is designed for rapid growth of E. coli and high yields of plasmid DNA.

Product Overview
_x000D_ At the end of log phase, cells growing in Circlegrow begin to utilize a proprietary second carbon source that becomes available on a delayed basis. Cell growth and plasmid replication continue long after cells in conventional media have reached stationary phase or expired. Bacteria commonly grow in CIRCLEGROW to a density triple that achieved in other media. The slow post-log phase growth of bacteria in Circlegrow eliminates the need for constant OD monitoring. Circlegrow cultures left overnight or over the weekend will have a much lower percentage of dead cells, and a higher percentage of plasmid DNA, than cultures prepared with any other media. The result is a higher yield of plasmid DNA in less volume with less attention.

Product Description
_x000D_ Circlegrow media(capsules) is an encapsulated growth media for growth of E.coli and extends plasmid production past log phase.

Recommended Use
_x000D_ Add 40 capsules per liter of purified water.

Typical Working Concentration
_x000D_ 40 g/L